RNA-seq of mouse Embryonic Stem Cell (mESC) line-derived endothelial and hematopoietic cells subjected to differential doxycycline (dox)-induced expression of transcription factors (TFs) in a Tal1?/? genetic background

RNA-seq of endothelial (Endo), Pre-hematopoietic progenitor cells (Pre-HPCs) and hematopoietic progenitors (HP) derived from the in-vitro hematopoietic differentiation of mouse Embryonic Stem Cells (mESCs) harboring a biallelic inactivating deletion of the endogenous Tal1 gene, and a construct for the dox-induced expression of the hematopoietic genes Tal1, Lyl1 and Lmo2 (i3TFs Tal1?/? mESCs). i: inducible. TFs: transcription factors. Cells were treated with dox at one (Dox Unt) or two (Dox Dox) time-points during hematopoietic differentiation to inducibly express the 3TFs. Sequenced cell populations were purified by FACS sorting based on the cell-surface expression of the endothelial marker VE-CADHERIN and the hematopoietic marker CD41. Endo: VE-CADHERIN+CD41- Pre-HPCs: VE-CADHERIN+CD41+ HP: VE-CADHERIN-CD41+"