Transcriptome assemblies of three diatom and three prymnesiophyte isolates from Station ALOHA and Kaneohe Bay

Culture ID/name

AT125A – Pseudo-nitzschia sp.

AT125C – Pseudo-nitzschia sp.

ATCH2 – Chaetoceros sp.

Pn B2 – Pseudo-nitzschia sp.

KB-HA01 – Chrysochromulina sp. (also called  

AL-TEMP-12 – Chrysochromulina sp. (also called 

NF-H275 – Chrysochromulina sp.

Growth Conditions

All cultures were grown at 27°C, 12:12 light:dark cycle, and with a light intensity of 100 µmol photons m-2 sec-1. AT125C, AT125A, and Pn B2 were grown with Aquil media. ATCH2 was grown with F/20 media with the phosphate concentration modified to a final concentration of 0.5µM. KB-HA01 was grown with F/2 media and AL-TEMP-12 and NF-H275 were grown with K media. None of the cultures were axenic. All cultures were filtered in “light” and “dark” conditions and were in exponential phase when filtered. (Filter types and volumes filtered listed below.) After filtration, all filters were placed into 2mL screwcap tubes, flash frozen with liquid nitrogen, and stored at -80°C.

Growth Conditions

All cultures were grown at 27°C, 12:12 light:dark cycle, and with a light intensity of 100 µmol photons m-2 sec-1. AT125C, AT125A, and Pn B2 were grown with Aquil media. ATCH2 was grown with F/20 media with the phosphate concentration modified to a final concentration of 0.5µM. KB-HA01 was grown with F/2 media and AL-TEMP-12 and NF-H275 were grown with K media. None of the cultures were axenic. All cultures were filtered in “light” and “dark” conditions and were in exponential phase when filtered. (Filter types and volumes filtered listed below.) After filtration, all filters were placed into 2mL screwcap tubes, flash frozen with liquid nitrogen, and stored at -80°C.

[TRANSCRIPTOME SEQUENCING]

[QC AND ASSEMBLY]

[POST-ASSEMBLY PROCESSING]
Diamond v2.0.5.143 was used to blast (e-value: 1e-5) to a cross-kingdom reference sequence database (as described in Coesel et al., 2021). Diamond v2.0.5.143 was used to find the least common ancestor of each contig based upon the blast results. Contigs that were identified as bacteria, archaea, or viruses were excluded from the assemblies.