Effect of biome depletion on the humoral immune response.

Antibody levels were determined by ELISA, except anti-muscle antibodies, which were determined by immunoblotting. The means and standard deviations are shown. 1 The p-values were determined using a t-test for normally distributed data indicated by a or a Mann-Whitney test for non-normally distributed data indicated by b (see Methods). Significant values (< 0.0500) are shown in bold. The p-values shown for IgG subclasses are post hoc tests run after a 2-way ANOVA that showed no significant effect of the state of the biome on IgG subclass concentrations. Effect of biome depletion on the humoral immune response.