Acetylome and Proteome from Human Adipose Tissue
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Characterization human adipose tissue acetylome in obesity and insulin resistance conditions. Lean individuals (Lean: BMI < 25; Glu < 100 mg/dL; HbA1c < 5,7%), obese normoglycemic patients (Ob-NG: BMI < 40; Glu < 100 mg/dL; HbA1c < 5,7%) and obese insulin resistance (Ob-IR: BMI < 25; Glu < 100-125 mg/dL; HbA1c > 5,7%). Samples were prepared to protein enrichment and analysed using a Dionex Ultimate 3000 nano-LC system in tandem with a linear quadrupole ion trap-Orbitrap mass spectrometer equipped with a nanoelectrospray ion source.
肥胖与胰岛素抵抗状态下人类脂肪组织乙酰化蛋白质组（acetylome）的表征分析。本研究纳入三类受试者：瘦体型个体（瘦组：体重指数BMI<25；血糖Glu<100mg/dL；糖化血红蛋白HbA1c<5.7%）、血糖正常肥胖患者（Ob-NG组：BMI<40；Glu<100mg/dL；HbA1c<5.7%）以及合并胰岛素抵抗的肥胖患者（Ob-IR组：BMI<25；Glu处于100~125mg/dL区间；HbA1c>5.7%）。所有样本经蛋白质富集处理后，采用Dionex Ultimate 3000纳流液相色谱（nano-LC）系统，与配备纳电喷雾离子源的线性四极杆离子阱-轨道阱（Orbitrap）质谱仪联用进行分析。
