Next Generation Sequencing of BAL cells from cadmium exposed mice

To understand the bioenergetics in cadmium-exposed macrophages, RNA sequencing (RNA-seq) was performed in lung macrophages isolated from vehicle- and cadmium-exposed mice. 8- to 12-week-old male and female C57/BL6 mice were intratracheally administered 100 ng/kg of CdCl2 or saline, as a vehicle control, after being anesthetized with 3% isoflurane using a precision Fortec vaporizer. Mice were euthanized 7 days after exposure and BAL was performed.