Base editor induced mutation spectrum in GFP, human ATP1a1, human TP53BP1 and murine MyoD. Base editor induced mutation spectrum in GFP, human ATP1a1, human TP53BP1 and murine MyoD

Analysis of targeted genomic and epigenomic mutations within GFP, human ATP1a1, human TP53BP1 and murine MyoD induced through state-of the art base editors Overall design: Genomic DNA was isolated from human HEK293T cells which constiutively express GFP or murine 3T3 cells which were transfected with the base editor and a gene-specific gRNA. Specific gene locus was amplified with barcoded primers and subjected to amplicon deep sequencing with either through PacBio Sequencing or Illumina Sequencing