CircMAN1A2 is upregulated by Helicobacter pylori and promotes development of gastric cancer

In this study, we treated the gastric cancer cell line AGS with PBS and Helicobacter pylori to perform RNA-seq analysis. A total of 18,308 different circRNA candidates were obtained in the experiment.Compared with the control, 101 significantly differentially expressed circRNAs were identified in the AGS cells infected with H. pylori, including 84 upregulated circRNAs and 17 downregulated circRNAs.Then, circMAN1A2 with the most significant expression difference was selected according to the sequencing results to study the epigenetic mechanism of H. pylori-induced gastric carcinogenesis. Overall design: After Hp26695-infected AGS cells were infected at a multiplicity of infection (MOI) = 100:1 for 12 h, gastric cancer cells were sequenced. The control group used PBS for the same amount of time.