Data and analysis for: Abiotic characteristics and organic constituents of oviposition sites that attract gravid female stable flies, Stomoxys calcitrans, and induce oviposition

This dataset is supplementary to the manuscript "Abiotic characteristics and organic constituents of oviposition sites that attract gravid female stable flies, Stomoxys calcitrans, and induce oviposition." This manuscript investigated the attractiveness and oviposition induction ability of several substrates and aimed to determine the key constituent(s) of a highly effective one. Prior to opening any subfolders, use the .Rproj file titled "Oviposition paper data publication.Rproj" to open RStudio and run all the subsequent .R files. Included in this dataset are: 1) raw data for numbers of flies attracted to the various substrates in head-to-head comparisons, 2) raw data for the numbers of eggs laid on the various substrates, 3) the Rcode used to convert the count data into proportions which were then compared for each experiment, and in some cases, across experiments, as well as to generate figures 2-7 in the text, and 4) the resulting figures 2-7. The "Read me.txt" files in each subfolder contain more detailed information for each set of experiments. Note: please refer to the "Read me.txt" file in the main folder for full descriptions of each subfolder. Article Abstract: "Gravid female stable flies, Stomoxys calcitrans (Linnaeus) (Diptera: Muscidae), oviposit in many types of organic substrates, including animal feces, but there is limited information as to which factors mediate attraction and oviposition. Here, we (1) tested effects of oviposition site moisture and odor on attraction and oviposition by flies, and (2) selected a highly effective oviposition site (fly rearing medium) to determine the key constituent(s) that mediate(s) attraction and oviposition. In moving- and still-air olfactometers as well as large laboratory rooms, we show that (i) odor and moisture of oviposition sites play distinguishable functional roles in the close range attraction of gravid female flies and their propensity to oviposit, (ii) rearing medium containing fish food, wheat bran, wood chips and a watery solution of ammonium bicarbonate (releasing NH3 and CO2) is a more appealing oviposition site to female flies than cow feces, (iii) ammonium bicarbonate in this medium is the key constituent for stable fly attraction and oviposition, (iv) NH3 alone or in combination with CO2, but not CO2 alone, attracts stable flies and induces oviposition, and (v) NH3/CO2 and fish food in combination are more attractive than NH3/CO2 or fish food alone. With fecal bacteria reportedly emitting NH3, and with stable fly larval development reportedly reliant on (fecal) microbes, it follows that gravid female flies may be guided by airborne microbe-derived cues originating from prospective oviposition sites. Isolating these microbes and identifying their odorants could enable the development of a synthetic odor blend which, coupled with NH3 and CO2, may prove highly effective as a trap lure to capture gravid female stable flies."本数据集为配套研究论文《吸引孕卵雌性厩螫蝇（Stomoxys calcitrans）产卵的产卵位点的非生物特性与有机组分》的补充数据。该论文探究了多种基质对厩螫蝇的吸引活性及产卵诱导能力，旨在明确高效产卵位点的关键组分。 在打开任何子文件夹前，请先使用名为“Oviposition paper data publication.Rproj”的R项目文件打开RStudio，并运行后续所有.R脚本文件。 本数据集包含以下内容：1）不同基质间头对头对照试验中吸引到的厩螫蝇数量原始数据；2）各基质上产出的虫卵数量原始数据；3）用于将计数数据转换为比例数据的R代码，该比例数据可用于单次试验及部分跨试验的结果比较，同时可用于生成正文中的图2至图7；4）生成得到的图2至图7。各子文件夹中的“Read me.txt”文件包含对应试验组的详细说明。 注意：请查阅主文件夹中的“Read me.txt”文件，以获取各子文件夹的完整说明。 ### 论文摘要 孕卵雌性厩螫蝇（*Stomoxys calcitrans* (Linnaeus)，双翅目：蝇科）可在多种有机基质中产卵，包括动物粪便，但目前关于调控其产卵位点选择与产卵行为的关键因子的相关研究仍较为有限。本研究（1）探究了产卵位点的湿度与气味对厩螫蝇吸引及产卵行为的影响，（2）选取一种高效产卵位点（蝇类饲养培养基），以明确调控其产卵位点选择与产卵行为的关键组分。 在流动空气与静止空气嗅觉仪以及大型实验室空间中，本研究结果表明：（1）产卵位点的气味与湿度在孕卵雌性厩螫蝇的近距离吸引及产卵倾向中发挥着可区分的功能性作用；（2）含有鱼粉、麦麸、木屑以及碳酸氢铵水溶液（可释放氨气NH₃与二氧化碳CO₂）的饲养培养基相较于牛粪更受雌性厩螫蝇青睐，可作为优质产卵位点；（3）该培养基中的碳酸氢铵是调控厩螫蝇吸引与产卵行为的关键组分；（4）单独使用氨气或氨气与二氧化碳联用均可吸引厩螫蝇并诱导其产卵，而单独使用二氧化碳则无此效果；（5）NH₃/CO₂与鱼粉联用的吸引效果优于单独使用NH₃/CO₂或单独使用鱼粉。 已有研究表明粪便细菌可释放氨气，且厩螫蝇幼虫的发育依赖于（粪便来源的）微生物，由此推测孕卵雌性厩螫蝇可能通过感知来自潜在产卵位点的空气传播微生物源信号来选择产卵位点。分离此类微生物并鉴定其释放的气味物质，有望开发出合成气味混合物，将其与NH₃和CO₂联用，可作为高效的诱捕诱饵用于捕捉孕卵雌性厩螫蝇。