Removal of glycerol from enzymatically produced 2-α-D-glucosylglycerol by discontinuous diafiltration

We provide here the underlying data of the scientific publication "Removal of glycerol from enzymatically produced 2-α-D-glucosylglycerol by discontinuous diafiltration". Please find the abstract below: 2-α-D-Glucosyl-glycerol (2-GG) is a natural glycoside that acts as a biological extremolyte. 2-<br> GG is a promising ingredient for cosmetic products. Industrially, 2-GG is synthesised from<br> sucrose in the presence of a high excess of glycerol using the enzyme sucrose<br> phosphorylase. For commercial applications, the surplus glycerol must be removed and an<br> efficient purification strategy is therefore demanded. Here, we demonstrate nanofiltration for<br> the separation of glycerol from 2-GG. Based on membrane screening performed at varied<br> pressure and temperature, we identified a 150 – 300 Da cut-off polyamide membrane for the<br> selective removal of glycerol at high permeate throughput. An enzymatically produced 2-GG<br> solution was purified with multicycle discontinuous diafiltration on the litre scale. The process<br> was operated at constant permeate flux (13 kg m² h-1) and at a temperature of 30 °C. A<br> complete removal of glycerol was achieved after six diafiltration cycles. This was in<br> accordance with the simulation results of a mass balance model. The purity of the product<br> solution increased to 80%, which is comparable with commercially products containing<br> functional carbohydrates. The achieved product recovery was around 84%.