The m6A-mediated TGF-β-SMAD signaling axis regulates ccRCC progression [LACE-seq]

TGF-β-SMAD signaling pathway plays an important role in progression of various tumors. However, posttranscriptional regulation such as N6-methyladenosine (m6A) of TGF-β-SMAD signaling axis remains incompletely understood. Here, we reveal that Insulin like growth factor 2 mRNA binding protein 2 (IGF2BP2) is low expression as well as associated with poor prognosis in clear cell renal cell carcinoma (ccRCC) patients and inhibits proliferation as well as promotes metastasis of ccRCC cells. To explore the underlying mechanism of IGF2BP2 in ccRCC, RNA-seq, RIP-seq and LACE-seq were performed to investigate the downstream targets of IGF2BP2. Comparative gene expression profiling analysis of RNA-seq data for sh-IGF2BP2 and Anti-IGF2BP2 for RIP-seq and LACE-seq in 786-O cells.