Number of reads and assignation rate for libraries enriched with single probe.

The total number of reads and assignation results against COI database is reported at both sites: Marañon and Napo rivers and for both samples: Mock and Bulk. For each sample the number of larvae is given. “Reads with hit” indicates the number of reads mapped to the COI database but not taxonomically assigned because the threshold filters (min bit-score 230 and identity 99%) were not met. “Reads assigned” accounts for the number of reads where taxonomic assignation was successful. Capture efficiency was estimated through the X-fold enrichment and calculated as the ratio of the percentage of reads with hit between an enriched and an unenriched library (see text for details).