Table 1 in Montichneumon, a new genus of Ichneumoninae (Hymenoptera, Ichneumonidae) from the high mountains of Taiwan

Table 1. PCR primers and conditions used in this study. Regarding PCR conditions, 35 cycles were run for COI, whereas 30 cycles were run for 28S. Abbreviation: PCR= polymerase chain reaction. MarkersPrimersPCR primers Sequences (5′–3′)ReferencesCOIFol_Ich_FTTTCAACAAATCATAAAGATATCGGFol_Ich_RTAAACTTCAGGATGACCAAAAAATCAChen et al. (2023)LCO1490GGTCAACAAATCATAAAGATATTGGHCO2198TAAACTTCAGGGTGACCAAAAAATCAFolmer et al. (1994)Primer Mix FLCO1490: Fol_Ich_F = 1:1Primer Mix RHCO2198: Fol_Ich_R = 1:1This study28S(F)GCGAACAAGTACCGTGAGGG(R)TAGTTCACCATCTTTCGGGTCLaurenne et al. (2006)PCR conditionsMarkersPrimary denaturationDenaturation Annealing ExtensionAdditional extensionCOI95°C, 4 min95°C, 30 sec 47°C, 1 min 72°C, 45 sec72°C, 10 min28S95°C, 4 min96°C, 15 sec 50°C, 30 sec 72°C, 30 sec75°C, 7 min