Shh Phosphatome Screen

Table 1-Phosphatome Screen-Shh Signaling. The individual genes tested in primary screen for phosphatases involved in Shh signaling. Gene symbol, gene name and identifier and the target sequence are shown. Four or five target sequences were used for each gene. Table 2- Phosphatome Screen. Individual genes were identified as positive hits in an shRNA screen for phosphatases that affect Shh signaling in ShhlightII cells as assessed by Gli promoter-luciferase expression. Table 5- Phosphatome Screen-Primary Data. Results of phosphatome screen for Shh signaling in ShhlightII cells. For each targeting sequence the gene targeted, the average value for Firefly/Renilla luciferase, the In of that ratio and the robust z score are shown. Each sequence was tested in control conditions (vehicle), with SAG stimulation (300nM) and with Shh stimulation (300ng/ml) and results for each are shown.