Crystal structure of 5beta-reductase (AKR1D1) E120H mutant in complex with NADP+ and epiandrosterone

Crystal structure of 5beta-reductase (AKR1D1) E120H mutant in complex with NADP+ and epiandrosterone Descriptor: (3Beta,5alpha)-3-Hydroxyandrostan-17-one, 3-oxo-5-beta-steroid 4-dehydrogenase, 5-ALPHA-ANDROSTANE-3-BETA,17BETA-DIOL, ... Authors: Chen, M, Christianson, D.W, Penning, T.M. Deposit date: 2011-12-07 Release date: 2012-03-21 Last modified: 2023-09-13 Method: X-RAY DIFFRACTION (1.637 Å) Cite: Conversion of Human Steroid 5beta-Reductase (AKR1D1) into 3β-Hydroxysteroid Dehydrogenase by Single Point Mutation E120H: EXAMPLE OF PERFECT ENZYME ENGINEERING. J.Biol.Chem., 287, 2012

与烟酰胺腺嘌呤二核苷酸磷酸（NADP+）和表雄酮（epiandrosterone）结合的5β-还原酶（AKR1D1）E120H突变体的晶体结构。描述符：(3β,5α)-3-羟基雄甾烷-17-酮、3-氧代-5β-甾族4-脱氢酶、5α-雄甾烷-3β,17β-二醇…… 作者：Chen M、Christianson D.W、Penning T.M。存档日期：2011-12-07；发布日期：2012-03-21；最后修改日期：2023-09-13。实验方法：X射线衍射（分辨率1.637埃）。引用文献：《单点突变E120H将人类固醇5β-还原酶（AKR1D1）转化为3β-羟基类固醇脱氢酶：完美酶工程的范例》，载于《生物化学杂志》（J.Biol.Chem.），2012年，第287卷。