Transcriptome-wide polymorphisms of red abalone (Haliotis rufescens) reveal patterns of gene flow and local adaptation

Global climate change is projected to accelerate during the next century, altering oceanic patterns in temperature, pH and oxygen concentrations. Documenting patterns of genetic adaptation to these variables in locations that currently experience geographic variation in them is an important tool in understanding the potential for natural selection to allow populations to adapt as climate change proceeds. We sequenced the mantle transcriptome of 39 red abalone (Haliotis rufescens) individuals from three regions (Monterey Bay, Sonoma, north of Cape Mendocino) distinct in temperature, aragonite saturation, exposure to hypoxia and disease pressure along the California coast. Among 1.17 × 10^6 Single Nucleotide Polymorphisms (SNPs) identified in this study (1.37% of the transcriptome), 21 579 could be genotyped for all individuals. A principal components analysis concluded that the vast majority of SNPs show no population structure from Monterey, California to the Oregon border, in corrobora...预计全球气候变化将在未来百年内加速，进而改变海洋的温度、pH值与溶解氧浓度格局。对当前存在上述环境变量地理差异的区域内，生物针对这些变量的遗传适应模式进行记录，是理解自然选择助力种群随气候变化完成适应的潜力的重要研究手段。本研究对加利福尼亚沿岸三个环境特征存在显著差异的区域（蒙特雷湾、索诺玛、门多西诺角以北）的39只红鲍（*Haliotis rufescens*）个体的外套膜转录组进行了测序，这些区域在海水温度、文石饱和度、低氧暴露与病害压力方面各不相同。本研究共鉴定出1.17×10^6个单核苷酸多态性（Single Nucleotide Polymorphisms，SNPs），约占转录组序列的1.37%，其中21579个位点可对所有受试个体完成基因分型。主成分分析（Principal Components Analysis）结果显示，从加利福尼亚州蒙特雷至俄勒冈州边界的绝大多数SNP位点均未表现出种群遗传结构，研究结果佐证了……