ITS restriction fingerprinting and direct sequencing of ITS regions and 26S rDNA D1/D2 domains.

Clusters G-1 to G-9 and singleton ITS profiles (-) are determined based on restriction digestion patterns of ITS amplicons with the endonucleases HaeIII, HhaI and HinfI. Restriction fragments lower than 70 bp are omitted from the analysis. Strains in bold are chosen as representatives of each cluster and/or unique ITS genotypes and are submitted to direct ITS and D1/D2 sequencing. Overlapping peaks in chromatograms due to ambiguous sites and/or indels are indicated as unreadable sequences. Closest-sequence matches are reported together with identity (%). Abbreviations: na, not applicable; nd, not determined; cp, copy.