The specific activity towards glutaryl-Ala-Ala-Phe-MNA cleavage is reduced in NEP mutants.
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All assays were conducted at 37°C at in 20 mM MES buffer, pH 6.5. ND = not determined. *The concentrations of NEPS546A and NEPS546T were too low to quantify, however, they were active enough to determine Km.
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2015-12-02
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