Linking mosquito ecology, traits, behavior and disease transmission

Supporting information and data used to produced Figure 3 of the manuscript of the same title, published in Trends in Parasitology. We analyzed the publication trends in mosquito research from 1900-2019. We used the Web of Science global citation database to obtain the list of publications between 1900 and 2019 that had reported effects of environmental factors (both abiotic and biotic) on life-history and behavior of mosquitoes. Our search included abiotic factors such as temperature, precipitation and humidity and biotic factors such as predation, larval nutrition and competition (both intra- and interspecific). The keywords ‘development time’, ‘mass’, ‘size’, ‘longevity’, ‘survival’, ‘egg’, ‘fecundity’, and ‘life history’ were used to identify literature on larval and adult mosquito traits. Similarly, the keywords ‘behavior’, ‘host seek’, ‘host preference’, ‘blood feed’, ‘oviposition’, and ‘activity’ were used to collate research articles on behavior of adult mosquitoes. Variants of every keyword (hyphenated versus non hyphenated, British versus American spelling differences, etc.,) were carefully taken into consideration to ensure the accuracy of search results. Every publication identified by the Web of Science search was scored based on the number of traits and behaviors reported. These publication-wise scores were then used to calculate the cumulative score for every mosquito trait and behavior listed above. These cumulative scores determine the width of each of the lines (connecting the independent and dependent variables) depicted in the connectome (see figure in the article). The list of publications we fetched in our search is available in two spreadsheets listed below. We analyzed the data and visualized the connectome using the circlize package in R software v3.4.2. Complete datasets and R code are contained in the 3 files enclosed: 1. Larval and adult traits: S3_Larval_adult_traits.csv 2. Adult behavior: S3_Adult_behavior.csv 3. R code: S3_Code.R本数据集用于支撑发表于《Trends in Parasitology》（《寄生虫学趋势》）的同标题论文图3的相关辅助信息与数据。 本研究分析了1900年至2019年间蚊虫研究领域的文献发表趋势。我们依托Web of Science全球引文数据库（Web of Science），获取了1900年至2019年间所有报道环境因子（涵盖非生物与生物因子）对蚊虫生活史与行为产生影响的文献列表。本次检索涵盖的非生物因子包括温度、降水与湿度，生物因子则涵盖捕食作用、幼虫营养及种内、种间竞争。 我们以“发育时长”“体重”“体型”“寿命”“存活率”“卵”“繁殖力”与“生活史”为关键词，筛选涉及蚊虫幼虫与成虫性状的相关文献。同理，我们以“行为”“宿主搜寻”“宿主偏好”“吸血”“产卵”与“活动”为关键词，整理关于成虫行为的研究论文。我们充分考量了各关键词的各类变体形式（包括连字符与非连字符形式、英式与美式拼写差异等），以确保检索结果的准确性。 通过Web of Science检索得到的每一篇文献，均根据其报道的性状与行为数量进行计分。随后，我们基于单篇文献的计分结果，计算得到上述各类蚊虫性状与行为的累计得分。这些累计得分决定了本文所述连接组图（参见论文配图）中各连接自变量与因变量的线条宽度。本次检索获取的文献列表包含于以下两份电子表格中。 本研究使用R软件v3.4.2中的circlize包完成数据分析与连接组图可视化。完整数据集与R代码包含于以下3个附件文件中： 1. 幼虫与成虫性状：S3_Larval_adult_traits.csv 2. 成虫行为：S3_Adult_behavior.csv 3. R代码：S3_Code.R