Effect of stretching on inflammation in a subcutaneous carrageenan mouse model analyzed at single-cell resolution

Understanding the factors that influence the biological response to inflammation is crucial, due to its involvement in physiological and pathological processes, including tissue repair/healing, cancer, infections, and autoimmune diseases. We have previously demonstrated that in vivo stretching can reduce inflammation and increase local pro-resolving lipid mediators in rats, suggesting a direct mechanical effect on inflammation resolution. Here, we aimed to explore further the effects of stretching at the cellular/molecular level in a mouse subcutaneous carrageenan-inflammation model. Stretching for 10 minutes twice a day reduced inflammation, increased the production of pro-resolving mediator pathway intermediate 17-HDHA at 48h post carrageenan injection, and decreased both pro-resolving and pro-inflammatory mediators (e.g., PGE2 and PGD2) at 96h. ScRNAseq analysis of inflammatory lesions at 96h showed that stretching increased the expression of both pro-inflammatory (Nos2) and pro-reso..., All ultrasound data acquisition and measurements were performed by investigators blinded to intervention condition. Ultrasound images of the back were acquired under isoflurane anesthesia.  A high-frequency ultrasound scanner (Vevo 2100, Fujifilm VisualSonics, Toronto, Canada) in B mode with a 21 MHz transducer (MS 250) was used for optimal spatial resolution. A conductive gel was centrifuged for 5 minutes to remove air bubbles and spread over the skin. The transducer was stabilized with a clamp and mounted into an articulated arm to control the distance and the angle between the transducer and the skin surface. the transducer was oriented transversal or sagittal perpendicular to the skin of the back and centered on the lesion area. Total lesion area was calculated by averaging the lesion area measured at transversal and sagittal positions.
Flow cytometry
Inflammatory lesions were excised and minced in 5% FBS-DMEM, using a scalpel, then the suspension was filtered through a 70mm filter...., , # README

\#**For the Ultrasound area:**
\#Berrueta et al_Area measured by US (ultrasound) carrageenan inflammation 24-96h. The data is organized as follow: There are 6 columns, column A describe the code assigned to each individual animal. Column B represent treatment: 0=No stretch, 1= stretch. Column C refers to the time for the treatment: from 24h to 96h. Column D refers to the batch or group of samples. Column E correspond to the weight of the tissue at the time of euthanasia in mg. Column F represent the ultrasound area of the inflammatory lesion per each sample.

\#**For the flow cytometry data**
**Neutrophils and macrophage subpopulation**. Analysis was performed using Flow cytometry. The data set is organized as follow:
\#**Berrueta et al_neutrophils dynamics**: There are 9 columns, column A indicates the code assigned to each individual animal. Column B represent treatment: 0=No stretch, 1= stretch. Column C refers to the time for the treatment: from 24h to 96h. Column D refe...