The ubiquitin-associated domain of human thirty-eight negative kinase-1 flexibly fused to the 1TEL crystallization chaperone via a 2-glycine linker and crystallized at traditional protein concentration

The ubiquitin-associated domain of human thirty-eight negative kinase-1 flexibly fused to the 1TEL crystallization chaperone via a 2-glycine linker and crystallized at traditional protein concentration Descriptor: Transcription factor ETV6,Non-receptor tyrosine-protein kinase TNK1 Authors: Nawarathnage, S, Pedroza Romo, M.J, Smith, T, Bunn, D, Stewart, C, Doukov, T, Moody, J.D. Deposit date: 2022-03-01 Release date: 2023-03-08 Last modified: 2023-12-20 Method: X-RAY DIFFRACTION (2.1 Å) Cite: Fusion crystallization reveals the behavior of both the 1TEL crystallization chaperone and the TNK1 UBA domain. Structure, 31, 2023

将人源38负激酶1（human thirty-eight negative kinase-1，TNK1）的泛素相关结构域（ubiquitin-associated domain）通过双甘氨酸连接肽柔性融合至1TEL结晶分子伴侣（crystallization chaperone），并在常规蛋白质浓度下完成结晶。描述对象：转录因子ETV6（Transcription factor ETV6）、非受体酪氨酸蛋白激酶TNK1（Non-receptor tyrosine-protein kinase TNK1）。作者：Nawarathnage S、Pedroza Romo M.J.、Smith T、Bunn D、Stewart C、Doukov T、Moody J.D.。存档日期：2022年3月1日；发布日期：2023年3月8日；最后修改日期：2023年12月20日。实验方法：X射线衍射（X-RAY DIFFRACTION），分辨率2.1埃（Å）。引用文献：《融合结晶揭示1TEL结晶分子伴侣与TNK1泛素相关结构域的行为》，刊载于《Structure》，第31卷，2023年。