c-Kit POL II traveling ratio.

c-Kit traveling ratio values (as defined by the relative ratio of POL II density in gene ORF vs. promoter-proximal regions) were obtained by chromatin immunoprecipitation with POL II antibody, which recognizes the N-terminal region of the large subunit of POL II and binds POL II in a phosphorylation-independent manner and indicate the enrichment levels of c-Kit +5/−0.5 or +9,7/−0.5 regions relative to the control and the input samples (see also Figure 2 and 3 legends); IkWT: Ikaros wild type HPCs; IkHT: Ikaros heterozygote null HPCs; IkNULL: Ikaros homozygote null lin− HPCs; DMSO: Dimethyl sulfoxide-treated G1E2 cells (0.01% for 2 h); Fvp: Flavopiridol-treated G1E2 cells (100 nM for 2 h).c-Kit POL II traveling ratio.