Mus musculus Transcriptome or Gene expression

The alive liver cells of three mice in each group (WT and ISXKO) were dissociated according to the above procedure and pooled for further analysis. For a target recovery of 15,000 single cells, 10,000 live cells were loaded into the 10 Genomics Chromium Single Cell chips. Next, libraries were prepared using the Chromium Single Cell 3 GEM Library & Gel Bead Kit v3 (10 Genomics) according to the manufacturer's instructions and sequenced on an Illumina Novaseq 6000 with a sequencing depth of at least 100,000 reads per cell and 150-bp (PE150) paired-end reads.