Supplemental Information_Nemutlu et al., 2015

Re: Decline of Phosphotransfer and Substrate Supply Metabolic Circuits Hinders ATP Cycling in Aging Myocardium. Emirhan Nemutlu1,2, Anu Gupta1, Song Zhang1, Maria Viqar1, Ekhson Holmuhamedov3, Andre Terzic1, Arshad Jahangir3*, Petras Dzeja1*, PLOS ONE, 2015 <strong>Supporting Information</strong> <strong>S1 Table.</strong> Mean values of phosphometabolite dynamics in adult and aging atrial myocardium. Data are expressed as % of oxygen replaced/min and represented as mean ± SEM (n=6-8). Pi, inorganic phosphate; ATP γ-phosphoryl: phosphate at the gamma position of adenosine triphosphate; ADP β-phosphoryl, phosphate at the beta position of diphosphate; CrP, Creatine phosphate; G6P, glucose-6-phosphate; G1P, glucose-1-phosphate; G3P, glycerol-3-phosphate. Student’s t-Test was used to determine the significance between groups (p&lt;0.05). <strong>S2 Table.</strong> Mean values of first, second, third, and fourth 18O-atom incorporation into Pi signifying cycles of ATP/Pi exchange between ATP consumption and synthesis sites. Data are expressed as % of oxygen replaced/min and represented as mean ± SEM (n=10-17). Student’s t-Test was used to determine the significance between groups (p&lt;0.05). <strong>S3 Table.</strong> Mean values of energy metabolite turnover rates and activity of corresponding metabolic pathways. Data are represented as mean ± SEM, n=5-6. Phosphometabolites turnover times were calculated using the formula: pt(phosphometabolite)=(1-2-N)´p([18O]H2O), where pt(phosphometabolite) is a fraction of 18O-labeled phosphometabolite at given time t, N is equal to the number of turnover cycles observed during incubation period, and p([18O]H2O) is a fraction of 18O in media water as described in Materials and Methods. Abbreviations as in S1 Table. Student’s t-Test was used to determine the significance between groups (p&lt;0.05).