The Essential Rhodobacter sphaeroides RSP1056-RSP0847 (CenKR) Two-Component System Regulated Tol-Pal and Cell Envelope Biosynthesis

To determine the regulon of RSP1056-RSP0847 TCS (response regulator with DNA-binding domains) we used ChIP-seq to determine the genomic occupancy of RSP0847 in strains with a hyperactive (RSP0847(D56E)), WT, and low activity (?RSP1056, RSP0847(D56A)) TCS. We then correlated cenR binding sites with changes in global gene expression between WT and strains with a hyperactive (RSP1056(D56E)) or low activity (?RSP1056, RSP0847(D56A)) TCS in RNA-seq experiments. Overall design: Use ChIP-seq to identify CenR binding sites and RNA-seq to determine how CenR binding affects transcription and define the CenR regulon in Rhodobacter sphaeroides.