RNA Sequencing Facilitates Quantitative Analysis of Transcriptomes of H1 derived cells with or without SB431542 treatment at Day8 after human early hematopoietic differentiation .

Purpose: The goals of this study are to verify the inhibition of TGFb signaling is suffice to surpress the expression of MAGs in H1 cells during human early hematopoietic differentiation through comparing the transcriptome profilings in WT samples and samples with SB431542 treatment collected at day 8 after hematopoietic differentiation. Methods: mRNA profiles of hESC samples collected at day 8 after hematopoiesis differentiation were generated by deep sequencing using Illumina GAIIx. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays Conclusions: Inhibiting TGFb signaling is suffice to significantly surpressed the expression of MAGs. Overall design: Transcriptomes of H1 derived cells with or without SB431542 treatment at Day8 after human early hematopoietic differentiation