CIL:34521

The video is a representative movie of baceria incubated in DMEM for 3 h on top of an array of pilars of stiffness 500 pN/micron. The video is real time and shows the behavior of one or two bacteria for 23 s after 3 h of incubation. The video relates to CIL: 34522 and 34523, which show bacteria incubated in different media. The pilars are 3 micron apart center to center. The video is DIC microscopy with the focus on the top of the pilars. See the already deflected pillars around the bacterium and both transient and sustained pulls. This video corresponds to Video 2 from PLoS Biol. 2008 Apr 15;6(4):e87.本视频为在刚度为500皮牛每微米（pN/μm）的微柱阵列表面、经DMEM培养基孵育3小时的细菌的代表性成像片段。该视频为实时录制，展示了孵育3小时后1至2株细菌在23秒内的行为表现。本视频关联条目CIL:34522与CIL:34523，这两个条目展示了在不同培养基中孵育的细菌。微柱的中心间距为3微米。本视频采用微分干涉差显微镜（Differential Interference Contrast microscopy, DIC）成像，对焦于微柱顶端，可观察到细菌周围已发生偏转的微柱，以及细菌产生的瞬时与持续牵拉行为。本视频对应《PLOS Biology》2008年4月15日第6卷第4期e87文章中的视频2。