Role of OspC effectors in the IFN response

Interferons (IFNs) are vital weapons in the arsenal of immune mediators produced in response to infection. We showed that Shigella sonnei blocks signaling and interferon-stimulated genes (ISGs) downstream of all families of IFN via secreted effectors of the OspC family. Notably, Ospc1 and OspC3 strongly block the IFN response. This project aims at investigating the cellular signaling pathways that are impacted by OspC1 and OspC3 with or without IFN stimulation and at clarifying whether observed changes in ISGs expression are general or specific to some ISGs. Overall design: 293T cells expressing either empty GFP, OspC1 or OspC3 were treated or not with IFN beta overnight. Samples are provided as biological triplicates (experiments performed on different days), resulting in 3 vectors x 2 IFN treatments x triplicates =18 samples.