Zooplankton metabarcoding (DP1.20221.001)

This data product contains the quality-controlled, native sampling resolution data and metadata from NEON's aquatic zooplankton metabarcoding sampling protocol, as well as associated metadata provided by a contracted lab. Field samples are collected in the water column of lakes using the most appropriate sampler (vertical tow net or Schindler trap) for the depth of water at the same time and location as morphological taxonomy samples, preserved in ethanol in the field, and shipped to a contracting lab for processing and sequencing. For additional details, see the user guide, protocols, and science design listed in the Documentation section in this data product's details webpage. Queries for this data product return a downloadable data package with laboratory methods and DNA extraction, PCR amplification, and sequencing metadata for samples from the queried sites and date range. Queries for the expanded package will also include a table containing links to the raw sequence data files. Latency: The expected time from data and/or sample collection in the field to data publication is as follows, for each of the data tables (in days) in the downloaded data package. See the Data Product User Guide for more information. zoo_fieldData: 30 zoo_dnaExtraction: 240 zoo_markerGeneSequencing: 240 zoo_pcrAmplification: 240 zoo_dnaRawDataFiles: 240 zoo_metabarcodeTaxonomy: 240 inv_metabarcodeTaxonList: 240 inv_metabarcodeSummary: 240 inv_dnaExtractionStandard: 240 inv_markerGeneSequencingStandard: 240 inv_pcrAmplificationStandard: 240 inv_dnaRawDataFilesStandard: 240 inv_metabarcodeTaxonomyStandard: 240本数据产品包含经过质量管控的原生采样分辨率数据，以及源自美国国家生态观测站网络（NEON）水生浮游动物元条形码（metabarcoding）采样方案的元数据，同时涵盖签约实验室提供的附属元数据。 野外样本采集自湖泊水柱，根据水深选用适配的最优采样设备（垂直拖网或辛德勒捕集器（Schindler trap）），与形态分类学样本于同一时间、同一地点完成采集；样本在野外以乙醇固定保存，随后运送至签约实验室开展处理与测序工作。 如需获取更多详细信息，请参阅本数据产品详情网页的「文档」板块中所列的用户指南、采样方案及科学设计文件。 针对本数据产品发起的查询将返回可下载的数据包，内含对应查询站点与日期范围内的样本的实验室方法、DNA提取、PCR扩增及测序元数据。若查询扩展数据包，则返回结果还将包含一张指向原始序列数据文件的链接表格。 数据延迟：下载数据包内各数据表从野外数据和/或样本采集到数据公开的预期耗时（单位：天）如下，详细信息请参阅《数据产品用户指南》。 zoo_fieldData: 30 zoo_dnaExtraction: 240 zoo_markerGeneSequencing: 240 zoo_pcrAmplification: 240 zoo_dnaRawDataFiles: 240 zoo_metabarcodeTaxonomy: 240 inv_metabarcodeTaxonList: 240 inv_metabarcodeSummary: 240 inv_dnaExtractionStandard: 240 inv_markerGeneSequencingStandard: 240 inv_pcrAmplificationStandard: 240 inv_dnaRawDataFilesStandard: 240 inv_metabarcodeTaxonomyStandard: 240