Single_Nematode_R_D

Nematodes that have been cultured in-house will be implemented in low-input trials to generate long read, linked-read, complementary DNA and HiC libraries for individual nematodes. The Nuclei EXtraction by SONication (NEXSON) method will be trialled to disrupt the nematode cuticle, isolating nuclei that will be split and prepared for HiFi, linked-read and HiC sequencing. The development of this method has particular implications for our ability to generate chromosomal assemblies for free-living and parasitic nematode species which cannot be easily cultured.