Additional file 1 of A split influenza vaccine formulated with a combination adjuvant composed of alpha-d-glucan nanoparticles and a STING agonist elicits cross-protective immunity in pigs

Additional file 1: Figure S1. Nano11-SwIAV induced IL-4 and IL-6 cytokine gene expression in TBLN of vaccinated pigs. Five-week-old SPF pigs were administered twice ID/IM with NanoS100-SwIAV, Nano11-SwIAV or control NanoS100 and challenged at post-prime vaccination day 35 with SwIAV H1N1-OH7. TBLN samples collected and stored in RNAlater at DPC6 were analyzed for gene expression using SYBR Green qRT-PCR method. Data represent the mean value of 7-8 pigs ± SEM. Statistical analysis was performed by one-way ANOVA followed by Tukey’s post-test. Asterisk represents significant difference between indicated groups (*p < 0.05, **p < 0.01).