M Pentecost, G Otto, JA Theriot, MR Amieva (2011) CIL:25359, Canis lupus familiaris, epithelial cell. CIL. Dataset
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https://cildata.crbs.ucsd.edu/media/videos/25359/25359.zip
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Reorganization of cells around a site of cell extrusion monitored live by time-lapse differential interference contrast microscopy. The process of extrusion began at hour 1 of observation and continued for 40 min. Monolayer was imaged on a Zeiss Axiovert 200M microscope using a 40× dry differential interference contrast microscopy (DIC) objective. Images were collected by a charge-coupled device camera (CoolSNAP-HQ, Photometrics, Tucson, AZ) and stored digitally every 10 min. Image corresponds to Fig 4 and Video 1 in PLoS Pathog. 2006 Jan;2(1):e3. Epub 2006 Jan 27.
通过对细胞外排位点进行实时时间推移微分干涉对比显微镜监测,实现了细胞周围结构的重组。外排过程始于观察的第1小时,并持续了40分钟。使用蔡司Axiovert 200M显微镜和40倍干式微分干涉对比显微镜(DIC)对单层细胞进行成像。图像由电荷耦合器件摄像头(CoolSNAP-HQ,Photometrics,图森,亚利桑那州)采集,并以每10分钟数字化存储一次。图像对应于PLoS Pathog. 2006年1月;2(1):e3,发表时间为2006年1月27日。
提供机构:
CIL



