16S rRNA gene data for aerobic BTEX-degrading enrichments exposed to sulfonamide polyfluorinated substances in fire-fighting foams and transformation products

Per- and polyfluoroalkyl substances (PFASs) from aqueous film forming foams (AFFFs) can hinder bioremediation of co-contaminants, such as trichloroethene (TCE) and benzene, toluene, ethylbenzene, and xylene (BTEX). Anaerobic dechlorination can require bioaugmentation of Dehalococcoides and for BTEX, oxygen is often sparged to stimulate in-situ aerobic biodegradation. We tested PFAS inhibition to TCE and BTEX bioremediation by exposing an anaerobic TCE-dechlorinating co-culture, an aerobic BTEX-degrading enrichment culture, and an anaerobic toluene-degrading enrichment culture to n-dimethyl perfluorohexane sulfonamido amine (AmPr-FHxSA), perfluorohexane sulfonamide (FHxSA), perfluorohexane sulfonic acid (PFHxS), or non-fluorinated surfactant sodium dodecyl sulfate (SDS). The anaerobic TCE-dechlorinating co-culture was resistant to individual PFASs exposures but was inhibited by >1,000x diluted AFFF. FHxSA and AmPr-FHxSA inhibited the aerobic BTEX-degrading enrichment. The anaerobic to..., At the end of the BTEX enrichment experiments with 10 μM of each PFAS, 4 mL slurry samples were centrifuged at 10,000g for 10 minutes, decanted, and extracted for DNA using a DNeasy PowerSoil Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. Extracted DNA was stored at -80°C and sent to Novogene Corporation Inc. (Sacramento, CA, USA) for bacterial 16S rRNA amplification and sequencing. At Novogene Corporation Inc. (Sacramento, CA, USA), DNA concentrations were normalized to 10 ng/μL and amplified targeting the V3-V4 region (470bp fragment length).  At Novogene Corporation Inc. (Sacramento, CA, USA), DNA concentrations were normalized to 297 10 ng/μL and amplified targeting the V3-V4 region (470bp fragment length). The amplicons 298 were then assessed for quality on an Agilent 5400 Fragment Analyzer (Agilent, Palo Alto, CA, 299 USA) before library preparation and sequencing on an Illumina NovaSeq6000 PE250 platform 300 (Illumina, San Diego, CA, USA)., , # **16S rRNA gene data for aerobic BTEX-degrading enrichments exposed to sulfonamide polyfluorinated substances in fire-fighting foams and transformation products** [https://doi.org/10.5061/dryad.0k6djhb7j](https://doi.org/10.5061/dryad.0k6djhb7j) **Description of the data** The data reported consist of 16S rRNA gene sequences from an aerobic enrichment culture that is able to degrade benzene, toluene, ethylbenzene, and xylenes under aerobic conditions. The enrichment was developed from a PFAS fire-fighting foam impacted site. The microcosm experiments evaluated inhibition to BTEX biodegradation upon exposure of individual sulfonamide-PFAS present int fire-fighting foam as well as their transformation products, perfluoroalkyl sulfonates. **Folder Structure**: 1. Raw_16S_data (.zip): This zipped folder contains the following files: * Folders (named B1a, B1b, etc.,) corresponding to specific samples which contain FASTQ files (raw files, and primers, barcodes removed) (.fq). Th...水成膜泡沫灭火剂（AFFFs）中的全氟和多氟烷基物质（PFASs）会阻碍三氯乙烯（TCE）、苯、甲苯、乙苯、二甲苯（BTEX）等复合污染物的生物修复。厌氧脱氯过程需对脱卤球菌属（Dehalococcoides）进行生物强化；而针对BTEX的修复通常会通入氧气以刺激原位好氧生物降解。本研究通过将厌氧三氯乙烯脱氯共培养体系、好氧BTEX降解富集培养物以及厌氧甲苯降解富集培养物暴露于N-二甲基全氟己烷磺酰胺胺（AmPr-FHxSA）、全氟己烷磺酰胺（FHxSA）、全氟己烷磺酸（PFHxS）或非氟表面活性剂十二烷基硫酸钠（SDS）中，探究了PFAS对TCE和BTEX生物修复的抑制作用。厌氧三氯乙烯脱氯共培养体系对单一PFAS暴露具有耐受性，但会被稀释1000倍以上的AFFF所抑制。FHxSA与AmPr-FHxSA会抑制好氧BTEX降解富集培养物的活性。厌氧甲苯降解富集培养物……。 在添加了10 μM各PFAS的BTEX富集培养实验结束后，取4mL泥浆样品以10000g离心10分钟，倾去上清液，随后按照制造商说明书，使用DNeasy PowerSoil土壤基因组DNA提取试剂盒（凯杰，德国希尔登）提取DNA。提取得到的DNA保存于-80℃，并送往诺禾致源公司（美国加利福尼亚州萨克拉门托）进行细菌16S rRNA扩增与测序。在诺禾致源公司，将DNA浓度归一化至10 ng/μL，靶向V3-V4高变区（片段长度470bp）进行扩增。将DNA浓度归一化至10 ng/μL并靶向V3-V4高变区（片段长度470bp）进行扩增。随后使用安捷伦5400片段分析仪（安捷伦，美国帕洛阿尔托）对扩增子进行质量评估，之后在Illumina NovaSeq6000 PE250测序平台（Illumina，美国圣地亚哥）进行文库制备与测序。 # **针对消防泡沫及其转化产物中磺酰胺类多氟物质暴露的好氧BTEX降解富集培养物的16S rRNA基因数据** [https://doi.org/10.5061/dryad.0k6djhb7j] **数据说明** 本数据集包含的16S rRNA基因序列来自一株好氧富集培养物，该培养物可在好氧条件下降解苯、甲苯、乙苯与二甲苯。该富集培养物分离自受PFAS类消防泡沫污染的场地。本微宇宙实验评估了消防泡沫中存在的单一磺酰胺类PFAS及其转化产物——全氟烷基磺酸盐对BTEX生物降解的抑制作用。 **文件夹结构**： 1. Raw_16S_data (.zip)：该压缩文件夹包含以下文件： * 以B1a、B1b等命名的文件夹，对应特定样本，其中包含FASTQ格式文件（已去除引物与条形码的原始测序数据）（.fq格式）。