Gene expression pattern of IFU5 deleted Candida albicans cells

To elucidate the impact of IFU5 in Candida albicans, genome wide transcription profiling was performed in ifu5?/? mutant strain. Wild type and mutant cells were grown for 5 hours and RNA extracted from these cultures, followed by microarray profiling. Expression of six genes (EFG1, ALS3, SOD3, BMT4, COX2, NAD1) was validated by qPCR. Agilent Whole Genome Candida albicans 8x15k Gene Expression (AMADID: 026377) designed by Genotypic Technology privatted Limited, Bangalore , Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)