Evolution of Vertebrate Fin Patterning Systems and Staging Guide of the Epaulette Shark (Hemiscyllium ocellatum)

The work presented in this thesis identifies the gene, shox2, as the first molecular marker of apical fold inducing cells (AFICs), a specialised cell type previously discovered to be important in the formation of the fin-fold and fin-rays of finned vertebrates. Using genetic tools in zebrafish, along with multi-stage single cell RNA-sequencing of pectoral fins of zebrafish and elephant shark, I further characterised AFICs molecular profile, function and evolution. Finally, I provide a husbandry and MicroCT staging guide for the epaulette shark, an important model the Currie lab is advancing for the study of cartilaginous fish.