SAIMOS - Biological and Flow Cytometry data collected from sampling stations in South Australia, in November 2020

Flow cytometry data was collected in November 2020, in waters off South Australia, from the Upper Spencer Gulf (SAMUSG). The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (DCM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.Six main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), SAM2CP/B4 (35.168S, 135.418E) and SAM5CB/B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.Since July 2019 two additional BGC stations have been added to the SAIMOS sampling array: the SA Upper Spencer Gulf Mooring (SAMUSG, 33.111S, 137.709E) and the SA Gulf St Vincent Mooring (SAMGSV, 35.023S, 138.46E).

本数据集的流式细胞术（Flow Cytometry）数据采集于2020年11月，采样区域为澳大利亚南部外海的上斯宾塞湾（Upper Spencer Gulf，缩写SAMUSG）。本研究的总体目标为建立澳大利亚南部大陆架海域生态系统的基础背景资料，阐明上升流与高盐溢流出事件对微生物群落的影响，最终构建该区域的生物地球化学模型。
采样工作依托“恩格林号”（RV Ngerin）科考船开展，属于澳大利亚南部综合海洋系统（Southern Australian Integrated Marine System，缩写SAIMOS）项目的一部分。每一轮科考航次中，研究团队均对叶绿素荧光最大值层（Deep Chlorophyll Maximum，缩写DCM）的物理、化学及生物学特性开展调查。本数据集已采集超微型浮游植物、细菌与病毒的流式细胞术检测数据。
本研究共对6个核心采样站位完成采样：其中5个站位位于100米等深线处，分别为RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、SAM2CP/B4（35.168°S，135.418°E）及SAM5CB/B5（35.008°S，135.198°E）；剩余1个为位于袋鼠岛西南侧的远海站位B1（36.188°S，136.178°E）。
需注意的是，结合站位间距（14~25海里）、陆架中层海流的平均流速分量（0.01 m·s⁻¹）以及科考船的平均航速（9节）可推断，各站位采集的为不同水团样本。研究团队还偶尔于袋鼠岛国家参考站位（National Reference Station，缩写NRS，35.832°S，136.447°E），以及斯宾塞湾口锚泊站（SA Spencer Gulf Mouth Mooring，缩写SAM8SG，35.25°S，136.690°E，该站位为高盐溢流出事件发生区域）采集额外样本。
自2019年7月起，SAIMOS采样阵列新增2个生物地球化学（Biogeochemical，缩写BGC）站位：澳大利亚南部上斯宾塞湾锚泊站（SA Upper Spencer Gulf Mooring，缩写SAMUSG，33.111°S，137.709°E）与圣文森特湾锚泊站（SA Gulf St Vincent Mooring，缩写SAMGSV，35.023°S，138.46°E）。