A new Ni(II)-based coordination complex: crystal structure and anti-biofilm activity against <i>P. gingivalis</i> during periodontal diseases
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In the current study, by employment of a multiple N-containing ligand 6,6′-bis(1H-benzo[d]imidazol-2-yl)-2,2′-bipyridine (L) featuring both benzimidazole and bipyridyl groups, a new coordination complex with the chemical formula of [Ni(L)(H<sub>2</sub>O)(ipa)] (<b>1</b>, H<sub>2</sub>ipa = Iso-phthalic acid) has been successfully prepared by reaction of Ni(NO<sub>3</sub>)<sub>2</sub>·6H<sub>2</sub>O with the L and the H<sub>2</sub>ipa ligand in a mixed solvent of H<sub>2</sub>O and DMF. Then we use the grinding method to prepare the nanoparticles of complex <b>1</b> with the average size of 350 nm. Furthermore, the inhibitory effect of nano <b>1</b> on <i>P. gingivalis</i> biofilm formation during periodontal diseases was assessed. Firstly, the treatment ability of nano <b>1</b> on <i>P. gingivalis</i> biofilm formation during periodontal diseases was evaluated by crystal violet staining assay. Then, the relative expression level of the <i>hagB</i> and <i>hagC</i> genes in <i>P. gingivalis</i> after nano <b>1</b> treatment was measured with RT-PCR.
