Signaling induced biophysical disruption of repressed chromatin domains drives immune cell fate
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Chromatin immunoprecipitation and input controls using anti-H3S28ph in resting and stimulated naive B cells (1 hour stimulation with 10 µg/ml anti-CD40 (Thermo Fisher Scientific 16-0402-86), 10 µg/ml anti-IgM (Jackson ImmunoResearch 115-005-020) and 5 ng/ml IL-21 (R&D Systems 594-ML-010). Resting is denoted by 0 or U. Treated is denoted by 60 or T.
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2025-01-22
