Multi-omics analysis reveals the glycolipid metabolism response mechanism in the liver of Genetically Improved Farmed Tilapia (GIFT, Oreochromis niloticus) under hypoxia stress

Background: Dissolved oxygen (DO) in the water is a vital abiotic factor in aquatic animal farming. A hypoxic environment affects the growth, metabolism, and immune system of fish. Glycolipid metabolism is a vital energy pathway under acute hypoxic stress, and it plays a significant role in the adaptation of fish to stressful environments. In this study, we used multi-omics integrative analyses to explore the mechanisms of hypoxia adaptation in Genetically Improved Farmed Tilapia (GIFT, Oreochromis niloticus). Results: The 96 h median lethal hypoxia (96h-LH50) for GIFT was determined by linear interpolation. We established control (DO: 5 mg/L) groups (CG) and hypoxic stress (96h-LH50) groups (HG) and extracted liver tissues for high-throughput transcriptome and metabolome sequencing. A total of 581 differentially expressed (DE) genes and 1250 DE metabolites were detected between CG and HG, and were annotated using tools at the KEGG database. We verified the transcript levels of eigh...背景：水体中的溶解氧（Dissolved Oxygen, DO）是水产养殖领域至关重要的非生物因子。低氧环境会影响鱼类的生长、代谢及免疫系统。糖脂代谢是急性低氧胁迫下的核心能量通路，在鱼类适应胁迫环境的过程中发挥关键作用。本研究采用多组学整合分析手段，探究了吉富品系尼罗罗非鱼（Genetically Improved Farmed Tilapia, GIFT, Oreochromis niloticus）的低氧适应机制。 结果：通过线性插值法确定了吉富罗非鱼的96小时半数致死低氧浓度（96h-LH50）。本研究设置了对照组（溶解氧浓度：5 mg/L，CG）与低氧胁迫组（96h-LH50浓度，HG），提取肝脏组织开展高通量转录组与代谢组测序。在CG与HG组间共筛选得到581个差异表达（differentially expressed, DE）基因及1250个差异代谢物，并通过京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）数据库工具完成功能注释。本研究验证了8个基因的转录水平（原文此处为截断内容“eigh”）