Accumulation of 8-PN and naringenin in the GM and plasma after their dietary intake for 22 days.

Blood was collected from mice fed flavonoid (5.6 mmol flavonoid/kg diet) for 22 days, and then total body reflux was carried out. The GM was collected after the reflux. Plasma was prepared from the blood as described in the Materials and Methods section. Values of the total 8-PN and total naringenin indicate the sum of aglycone and conjugated metabolites obtained by the HPLC analysis with deconjugation treatment. Amount of flavonoids was determined by HPLC analyses as described in the Materials and Methods section. Data are the mean ± S.E. Different letters indicate significant differences upon analyses by the Tukey multiple comparison test with two-way ANOVA (p = 1.79×10–9).