Picophytoplankton and bacteria total carbon estimates from cell counts analyzed with flow cytometry (FCM) from CCE-CalCOFI Augmented cruises in the California Current System, 2004 - 2017 (ongoing).

Picophytoplankton populations and non-pigmented prokaryotes are sampled within the California Current Ecosystem (CCE) for abundances from 3 to 8 depths at CalCOFI stations. Seawater is collected from Niskin bottles and cells are fixed in the field aboard the survey cruises (since 2004, ongoing) with paraformaldehyde, and stained with a DNA-specific dye back in the laboratory. The cells are enumerated by an Altra flow cytometer (with a syringe pump for volumetric sample delivery) simultaniously with argon ion lasers, to distinguish three major populations of photoautotrophs (Prochlorococcus, Synechococcus, and pico-eukaryotes) and the assemblage of heterotrophic prokaryotes collectively referred to as H-Bact. FCM abundance estimates for each are converted to carbon biomass equivalents using mixed-layer estimates.本数据集在加州洋流生态系统（California Current Ecosystem, CCE）的CalCOFI站位处，于3至8个不同水深采集微微型浮游植物种群与无色原核生物样本以测定其丰度。海水通过尼斯金采水器（Niskin bottles）采集，自2004年起持续进行的调查航次中，研究人员在现场使用多聚甲醛（paraformaldehyde）固定细胞，后续在实验室中利用DNA特异性染料对细胞进行染色。搭载注射泵实现定量进样的Altra流式细胞仪配合氩离子激光器，对细胞进行计数，以此区分3类主要的光合自养生物种群——原绿球藻（Prochlorococcus）、聚球藻（Synechococcus）及微微型真核浮游生物，以及被统称为H-Bact的异养原核生物群落。通过混合层厚度估算值，将各类群的流式细胞术（Flow Cytometry, FCM）丰度估算值转换为碳生物量当量。