Tbr1+/- Mouse whole brain, cocktail treatment

Autism spectrum disorders (ASD) are a group of heterogeneous, behaviourally defined neurodevelopmental conditions influenced by both genetic and environmental factors. Here, we show that supplementation of low-dose multiple nutrients (an important environmental factor) can modulate synaptic proteomes, reconfigure neural ensembles, and improve social behaviours in mice. We first used Tbr1+/- mice, a well-established model of ASD, to investigate the effect of 1/4 cocktail (containing Ile 0.1125%, Leu 0.225%, Val 0.1125%, 1% serine and 20 ppm zinc) in drinking water. To define the total proteomes after seven days supplementation of nutrient cocktails treatment alters in mouse cortex by LC-MsMS.