Composition of the model after each step.

aAll ribosome-associated proteins are counted as a single complex. The number for protein complexes and isozymes is not on a reaction basis but rather a protein basis. bDistinct species ignoring the compartment of the metabolites. cIncludes minor adjustments to compartment assignment. dBased on 482 protein-encoding ORFs. eAfter additional BLASTP matches, specificity adjustments and conversion to computations-ready form.