NELF-A controls Drosophila healthspan by regulating heat-shock protein-mediated cellular protection and heterochromatin maintenance [RNA-seq]

Comparison of differentially expressed genes in Day 50 adult heterozygous NELF-A*/+ mutant (BX) and control sibling (Sib) fly heads (2 replicates). Read quality check was performed using FASTQC and adaptors were trimmed using Trim Galore. Reads were aligned to Drosophila reference genome (BDGP6.22.98) using STAR (v2.5). HTSeq-count was used to identify read counts per gene. Differentially expressed genes (DEGs) were determined using DESeq2 (v1.22.1) on Galaxy platform. The data was visualised by Heatmap2 (v3.0.1). Gene Ontology analysis was performed using DAVID Functional Annotation Bioinformatics (v6.8).