Original immunofluorescence images and quantification data supporting PI3K-Akt pathway analysis in microplastic-exposed DSS colitis

This dataset contains the original immunofluorescence TIFF exports and replicate-level quantification data supporting Figure 9, Supplementary Figure 8, and Supplementary Tables 1–2 of the related manuscript, “Microplastic exposure activates PI3K-Akt signaling in ulcerative colitis: integrated computational toxicology, RNA-seq, GeneMANIA network analysis, and in vivo validation.” The dataset includes p-AKT and p-PI3K staining of mouse colonic tissue from the normal control, DSS-colitis, and DSS-colitis + microplastics groups. For each marker and group, three biologically independent samples are provided. Each sample contains a DAPI image, a red target-channel image, and the corresponding merged image, yielding 54 original TIFF files. All TIFFs are 1920 × 980 pixels and contain 25-µm scale bars. The files were copied and renamed without pixel modification. A manifest links every deposited image to its publication panel, experimental group, biological replicate, fluorescence channel, original source filename, and SHA-256 checksum. Replicate-level Image-Pro Plus measurements and normalized-density values are also included. The p-AKT staining pattern was interpreted as epithelial-predominant based on tissue morphology. Because no epithelial cell-type-specific co-staining was performed, these images do not establish definitive cell-type localization.