Data from experiments testing the effects of hypoxia on behavior and physiology of two species of rockfish from from 2015-2016
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<p>Juvenile rockfishes were collected from shallow (~10-20 m depth) rocky reef and kelp forest habitats at Stillwater Cove, central California (36˚ 34' N, 121˚ 56' W) during May-June of 2015. Newly settled copper (Sebastes caurinus) and blue (Sebastes mystinus) rockfish were collected weekly using large mesh hand nets while SCUBA diving. Fishes were measured for length and weight and tagged using Visual Implant Elastomer Tags. Juvenile rockfishes were exposed to one of four treatment levels corresponding to conditions that currently occur or are predicted to occur in the future on the central California coast (Chan et al., 2008; Booth et al., 2012; Fig. 1): 100% saturation (8.74 ± 0.03 mg O2 L-1), 68% saturation (6.00 ± 0.04 mg O2 L-1), 46% saturation (4.06 ± 0.04 mg O2 L-1), or 26% saturation (2.25 ± 0.05 mg O2 L-1), with two replicate tanks per treatment level. Behavior and physiological trials were conducted on each individual, including (1) escape response, (2) behavioral lateralization, (3) standard metabolic rate, (4) maximum metabolic rate, (5) aerobic scope, (6) pCrit (i.e., hypoxia tolerance test), and (7) ventilation rate. These data are published in Mattiasen et al. (2020).</p>
<p>2015年5—6月，研究人员于加利福尼亚州中部静水区（Stillwater Cove，北纬36°34′，西经121°56′）水深约10~20米的岩礁与海带林生境中采集幼年岩鱼。研究人员采用水肺潜水配合大网目手网的方式，每周采集新定居的铜平鲉（*Sebastes caurinus*）与蓝平鲉（*Sebastes mystinus*）幼鱼。对采集到的个体测量体长与体重，并使用视觉植入弹性体标签（Visual Implant Elastomer Tags）进行标记。随后将幼年岩鱼暴露于四种溶氧处理梯度中，这些梯度对应加利福尼亚中部海岸当前及未来预测的溶氧环境（Chan等，2008；Booth等，2012；图1）：100%饱和度（8.74±0.03 mg O₂ L⁻¹）、68%饱和度（6.00±0.04 mg O₂ L⁻¹）、46%饱和度（4.06±0.04 mg O₂ L⁻¹）以及26%饱和度（2.25±0.05 mg O₂ L⁻¹），每个处理组设置两个重复水族箱。对每尾个体开展行为与生理学测试，涵盖（1）逃逸反应、（2）行为偏侧化、（3）标准代谢率、（4）最大代谢率、（5）有氧代谢范围、（6）pCrit（即低氧耐受测试）以及（7）通风率。本数据集相关研究成果发表于Mattiasen等（2020）。</p>
