Flow cytometry data for STT3B inhibitory activity of NGI-1 derivatives related to the study "STT3A is essential for Wnt signaling and represents a target for cancers driven by RNF43 deficiency"

To assess the STT3B inhibitory activity of NGI-1 derivatives, ER-mEGFP cells were seeded into 96-well plates at a density of 20,000 cells/well in 100 μL medium. After a 24-hour adhesion period, cells were treated with a 2-fold serial dilution of the indicated NGI-1 derivatives (final concentrations ranging from 62.5 nM to 2 μM) for 24 hours. mEGFP fluorescence was then measured by flow cytometry.