Skin microbiome of forehead in children with and without acne

Whole genome sequencing of 48 volunteers, males and females, ages 7-17 years of age with and without acne. Tanner staging was used to assess pubertal development, IGA score assessed acne severity. A 7cm2 area of the forehead was scrubbed with a pre-moistened sterile, synthetic fiber swab for 30 seconds. Positive and negative sampling controls also included. Sequencing was performed by Illumina NextSeq sequencing (2x150 paired end reads).