Time course of the Arabidopsis root stem cells

The goal of this dataset was to transcriptionally profile the Arabidopsis root stem cells over developmental time. To accomplish this, we protoplasted roots expressing PET111:GFP every 8 hours from 4 days to 6 days old. We then collected GFP negative cells from the PET111:GFP line using cell sorting, as these cells represent the stem cell population. RNA was extracted from cells, libraries were prepared, and RNA-seq was performed. FPKM values were calulcated using Cufflinks. Arabidopsis roots expressing PET111:GFP were protoplasted every 8 hours from 4 days to 6 days post-stratification. GFP-negative cells were collected, RNA was extracted, and RNA-seq was performed.