Additional file 16: of Survey of cryptic unstable transcripts in yeast

Primers Used for RT-qPCR. RT-qPCR primer names and sequences. Primers are specific to either S.cerevisiae (S.cere) or S.paradoxus (S.para), but in some cases could be used in either species background. All primers were named for the nearest or overlapping gene annotation. Those primers labeled “–T” denote the presence of the unique 5′ tagged used for strand-specificity in RT reactions. An asterisk in the final column denotes candidates not requiring strand-specific RT-qPCR; for these candidates qPCR was performed on random hexamer primed cDNA (see Methods). (XLSX 15 kb)