RNA-seq data of alveolar type 2 epithelial cell derived from organoids treated with IL-1b or TNFa

Treatment with IL-1b or TNFa enhance the proliferation of alveolar type 2 cells in organoid culture. Here, we examine the downstream mechanisms that enhance AEC2 proliferation after IL-1b and TNFa treatments, we performed RNA-seq analysis of AEC2s isolated from organoids 6 hrs after exposure to either cytokine. We identified 165 differentially expressed genes that were common in both IL-1b and TNFa treated AEC2s compared to saline treated controls. mRNA was isolated from FACS-sorted cells and sequenced using Illumina HiSeq X